Diagnostic accuracy of loop-mediated isothermal amplification (LAMP) for screening patients with imported malaria in a non-endemic setting
Diagnostic accuracy of loop-mediated isothermal amplification (LAMP) for screening patients with imported malaria in a non-endemic setting
Blog Article
Background: Sensitive and easy-to-perform methods for the diagnosis of malaria are not yet available.Improving the limit of ashley furniture porter b697-92 (porter 3-drawer nightstand) detection and following the requirements for certification are issues to be addressed in both endemic and non-endemic settings.The aim of this study was to test whether loop-mediated isothermal amplification of DNA (LAMP) may be an alternative to microscopy or real-time PCR for the screening of imported malaria cases in non-endemic area.Results: 310 blood samples associated with 829 suspected cases of imported malaria were tested during a one year period.Microscopy (thin and thick stained blood slides, reference standard) was used for the diagnosis.
Real-time PCR was used as a standard of truth, and LAMP (Meridian Malaria Plus) was used as an index test in a prospective study conducted following the Standards for Reporting Diagnosis Accuracy Studies.In the 83 positive samples, species identification was P.falciparum (n = 66), P.ovale (n = 9), P.vivax (n = 3) P.
malariae (n = 3) and 2 co-infections with P.falciparum + P.malariae.Using LAMP methods, 93 samples gave positive results, including 4 false-positives.Sensitivity, specificity, positive predictive armatrex value and negative predictive value for LAMP tests were 100%, 98.
13%, 95.51%, and 100% compared to PCR.Conclusion: High negative predictive value, and limit of detection suggest that LAMP can be used for screening of imported malaria cases in non-endemic countries when expert microscopists are not immediately available.However, the rare occurrence of non-valid results and the need for species identification and quantification of positive samples preclude the use of LAMP as a single reference method.